Research Data
Explore our protein structure data and research findings.
POP2 Protein Structure
Interactive 3D model of the human POP2 protein
3D Model
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Research Findings
Key insights from our POP2 expression optimization research
Expression Analysis
Our initial findings demonstrate successful expression of human POP2 in both bacterial and mammalian expression systems. The SDS-PAGE analysis confirms the presence of the target protein at the expected molecular weight of approximately 45 kDa.
Fig 1: SDS-PAGE analysis showing POP2 protein expression
Fig 2: SDS-PAGE gel electrophoresis of purified POP2 protein
Protein Concentration Analysis
We performed a standard curve analysis to determine the relationship between protein concentration and band intensity. This analysis allows us to accurately quantify the expression levels in different conditions.
| Protein Concentration (μg) | Band Intensity |
|---|---|
| 1000 | 7984392 |
| 500 | 3216150 |
| 250 | 2578960 |
| 125 | 2116530 |
| 62.5 | 875520 |
| 31.25 | 439432 |
| 15.625 | 186554 |
| 7.8125 | 63414 |
Key Takeaways
- Successful expression of human POP2 in both E. coli and mammalian cell systems
- Optimal expression conditions identified: 0.5mM IPTG induction at 18°C for 16 hours in E. coli
- Purification yielded approximately 8mg of protein per liter of bacterial culture
- Protein maintains proper folding and activity as confirmed by structural analysis
- Linear relationship between protein concentration and band intensity observed up to 500μg